DSM BIOLOGY LAB PREP

 

CONTENTS

BIO 141: General Biology Lab Manual by Helen Gjessing
BIO 142: General Biology Lab Manual by Helen Gjessing
NSC 102: Handouts

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BIO 141: General Biology Manual by Helen Gjessing

 

1

2A

2B

3

4

5

6

7

8

9

10

11

12

13

EXP. 1: THE MICROSCOPE

 

 

microscope slides

S111floor (carolina; k3-63-1920; 50+)

microscope cover slips (22x22)

S111 (42) (carolina; k3-63-2962; each)

pond water in dropping bottles

carolina; k3-L25 B; each/120

letter "e" slides

Box 25 (carolina; k3-BM1; each)

crossed threads slides

Box 25 (carolina; k3-BM10/12; each)

transparent vinyl ruler (6"x150mm)

S111 (21) (carolina; k3-70-2604; 12/pk)

distilled water in dropping bottles

S111 (43-45) 

lens paper in petri dishes (8x6)

S111 (39) (carolina; k3-63-4000; 50)

petri dishes (100x15mm)

S109 floor (carolina; k3-74-1350; 500/case)

cheesecloth

S111 (62) (carolina; k3-71-2690; 12/pk)

scissors

S111 (35)

wash bottle

S111 (3,4)

disposable dropping pipets, cal. 1ml

S111 (30) (carolina; k3-21-5840; 500/box)

Return to Contents Section
 

EXP. 2A: SOME CHARACTERISTICS OF ORGANIC COMPOUNDS

 

 

goggles, 1 per student

S201 floor 

sugar models

S112 (22) (carolina; k3-84-0400; per set

glucose, 10%(dextrose); 200ml

S111 shelf (carolina; k3-85-7450; 500g)

gelatin, 10% (200ml)

S111 shelf (carolina; k3-86-4660; 500g)

fructose, 10% (levulose); 200ml

S111 shelf (carolina; k3-87-2340; 100g)

galactose, 10% (200ml)

S111shelf (carolina; k3-86-4500; 100g)

sucrose, 10% (200ml)

S111shelf (carolina; k3-89-2870; 500g)

lactose, 10% (200ml)

S111 shelf (carolina; k3-87-1750; 500g)

maltose, 10% (200ml)

S111 shelf (carolina; k3-87-3750; 100g)

starch (potato), 10% (50ml)

S111 shelf (carolina; k3-89-2532; 500g)

glycogen (oyster), 10% (50ml)

S111 shelf (carolina; k3-86-5610; 5g)

distilled water, 200ml

S111 counter

parafilm to cover containers with sugars

S111 counter

IKI soln. for starch test

S111 shelf  (carolina; k3-86-9055; 500ml)

Benedict's reagent

S111 shelf  (carolina; k3-84-7121; 500ml)

milk

local store

cotton

S111 (16) (carolina; k3-71-2674; 2000/case)

potato

local store 

plantain

local store

peanuts

local store

apple

local store

vegetable oil

S111 shelf or S205: 05791

butter

local store or cold room

liver extract (liver extract conc, 1:20) 40 ml

S111 shelf (carolina; k3-87-2750; 50g)

acetone, reagent

S111 shelf (carolina; k3-84-1504; 20L)

sudan black stain (sudan black b soln.)

S111 shelf (carolina; k3-89-2935; 500ml)

10% gelatin, 10ml/test tubes

S111 shelf 

egg white (or Albumin) in test tubes

S111 shelf (carolina; k3-84-2252; 500g)

NaOH, 10% (in dropping bottles)

S111 shelf

copper sulfate, 20% (0.5% in drop  btls)

S111 shelf (carolina; k3-85-5641; 120ml)

brown paper cut into small squares

S205 counter

formaldehyde

S111 shelf

glycerol (200 ml)

S111 shelf or S205

microscope slides

S111 floor or teacher desks: S109 & S110

mircoscope cover slips (22x22)

S111(42)

razor blade or knife 

S111 counter

spatula,one

S111 (35)

hot plate, two

S111 counter 

test tube racks, (3) three

S109 shelf 

test tube clamps, (6) six

S111 (35) 

beaker w/ tap water, two 1000ml&two 250 ml;1/4 fill each beaker with tap water

S111 shelf

sterile dropping pipet, cal. 1 ml

S111 floor & teacher desks: S109 & S110 (carolina; k3-21-4552; 500/box)

toothpicks, flat, plain (for tasting sugars)

S111 (23)

rubber stoppers for test tubes, (6) six

S111 shelf 

disposable test tubes, small

S111 (46, 47)

Return to Contents Section
 

EXP. 2B: SOME CHARACTERISTICS OF ORGANIC COMPOUNDS

 

 

distilled water, (150ml in a 250ml beaker)

S111 shelf  & table top

ethanol, 0.1M (150ml in a 250ml beaker)

S111 shelf

acetic acid, 0.1M (150ml in a 250 ml beaker)

S111 shelf

NaCl, 0.1M (150 ml in a 250 ml beaker)

S111 shelf

HCl, 0.1M (150 ml in a 250 ml beaker)

S111 shelf

NaOH, 0.1M (150 ml in a 250 ml beaker)

S111 shelf

sucrose, 0.1M (150 ml ina 250 ml beaker)

S111 shelf

10% gelatin + 10% glucose, 10ml/test tubes

S111 shelf

pH  meter or pH paper

S111 (42)

pH buffer

S111 counter

beaker, 1000 or 600 ml to rinse electrode

S111 shelf

wash bottle w/ distilled water to rinse electrode

S111 shelf

sample bottle w/ distilled water, 300ml

S111 shelf

lamp filament (100 watt) with electrode

S112 (22)

Return to Contents Section
 

EXP. 3: TRANSPORT INTO AND OUT OF CELLS

 

 

microscope slides

see exp. 1

microscope cover slips (22x22)

see exp. 1

carmine dye or car. alum stain w/ formalin

S111 shelf  (carolina; k3-85-3111; 25ml)

potassium permanganate crystals 

S111 shelf (carolina; k3-88-4130; 500g)

dissecting needles

S111 (35) (carolina; k3-62-7220; 12/pk)

spatulas, 2 needed

S111 (35)

dialysis tubing, 50ft, 12K to 14K MW cutoff

S111 (39) (carolina; k3-68-4218; 50ft)

string

S111 (39)

scissors, 2 needed

S111 (35)

ruler, 6 inch (2 needed)

S111 (21)

glucose, 10% (2000ml needed)

S111 shelf

Elodea Leaves (Elodea tips)

Carolina or UVI pond (carolina; k3-15-7340; class/15

forceps, 3 needed

S111(35)

Calcium Chloride, 4% (5ml/student)

S111 shelf (carolina; k3-85-1800; 500g)

sterile lancets, (hypolet kit)

S112 (46,47) (carolina; k3-70-0507; per kit)

hypolet lancets

S112 (46,47)  (carolina; k3-70-0508; 100ct)

hypolet guards

S112 (46,47) (carolina; k3-70-0509; 100ct)

prep pads (alcohol preps)

S112 (46,47) (carolina;k3-70-0530;100ct)

gauze pads

S112 (46,47) (carolina; k3-70-0540;100ct)

normal saline, 0.9% (5ml/student)

S111 shelf 9 (carolina; k3-88-8933;500ml)

distilled water in dropping bottles

S111 shelf

osmotic membrane/dialysis membrane

S111 (39) (carolina; k3-68-4320;100 sheets)

distilled water, 1 gallon

S111 counter

beakers, 1000/600ml (four needed)

S111 shelf

disposable test tubes, small

S111 (46,47)

test tube racks, 3 needed

S109 shelf

rubber stoppers for test tubes, 6 needed

S111 shelf

nutrient agar plates

cold room  (carolina; k3-82-1860; 10 plates)

wash bottle w/ fresh water (may not be needed)

S111 shelf

wash bottle w/ distilled water

S111 counter

Label: Used Test Tubes

prepare for lab

Label: Used Slides and Cover Slips

prepare for lab

Label:New/Not Used Slides and Cover Slips

prepare for lab

Return to Contents Section
 

EXP. 4: AEROBIC RESPIRATION AND FERMENTATION

 

 

microscope slides

see exp. 1

micoscope cover slips (22x)

see exp. 1

bunsen burner

S109 drawer

disp innoculating loop/needle; 10uL

S111 (40) (carolina; k3-21-5832; 500/case)

disp innoculation loop/needle; 1uL

S111 (40) (carolina; k3-21-5830; 500/case)

litmus milk,uninnoculated (5ml in 10 test tubes)

S111 shelf (carolina; k3-78-4821; 500g)

E. Coli in litmus milk, 5ml in test tubes

S111 shelf (carolina; k3-15-5065A)

bacillus subtillis in litmus milk, 5ml in 10 test tubes

S111 shelf (carolina; k3-15-4921A)

streptococcus lactis in litmus milk, 5ml in 10 test tubes

S111 shelf (carolina; k3-15-5610A)

disposable gloves

teacher desks: S109 & S110 

respiration apparatus

S112 (42)

germinating pigeon or other peas

S111 shelf

place in container of water friday for tuesday class; and monday for thursday's lab

alternate dependent on lab days

KOH pellets

S111 shelf (carolina; k3-88-3470; 500g)

10 large test tubes (25x200mm)

S111 floor 

cotton plugs

S111 (160) (carolina; k3-71-2674; 2000/case)

rubber stoppers w/ capillary tubing

S111 floor 

glass beads

S111 shelf (carolina;ke-72-5406; per lb)

food coloring or dye

S111 shelf, local store

ring stand, 6 needed

S111 floor

10% bleach solution (disinfectant)

S111 sink

millimeter ruler, 2 needed

S111 (21)

tweezers, 2 needed 

S111 (35)

pinch clamps

S205 (31)

Return to Contents Section
 

EXP. 5: PHOTOSYNTHESIS

 

 

IKI solution in dropping bottle

S111shelf

benedict's reagent in droppong bottle

S111shelf

distilled water, 5ml/student

S111 counter

fresh spinach or other dark green leaf

Local

algae (green, red, and brown)

C,D, E, F, F2 (carolina; k3-15-1540; per set)

Denoyer-Geppert Chart # 1894: Algae

S110 floor

coleus treatment (24hrs light), need 4 plants

Box Q,S (carolina; k3-15-7310; each)

2 healthy green plants per class (24hrs darkness then 72hrs in bell jar) prepare plants friday for Tuesday class and Sunday for Thursday class; alternate dependent on lab days; NAOH solution treatment for one plant.

local store

2 bell jars, glass (7"ID, 11"hgt)

S111 shelf (carolina; k3-75-2982; each)

Elodea sprig/tips

carolina or UVI pond (carolina; k3-15-7340; class/15)

sodium bicarbonate NaHCO3,0.25%

S111 shelf (carolina; k3-88-8380; 500g)

razor blades, to cut leaves

S111 counter

hot plates, 2 needed

S111 counter

ring stand, 6 needed

S111 floor

beakers, six 250ml

S111 shelf

paper towels (C-fold)

teacher desks: S109 & S110

lamp (2)

S114

200 & 100 watt bulb (low watt preferred, i.e., 40watt or less)

S112 (48, 22)

petri dishes (100x15mm)

S109 floor (carolina; k3-74-1350; 500/case)

ethanol, 95% (25ml/student or 1 gal)

S111 shelf

ethanol, 70% (100ml needed TOTAL)

S111 shelf

cotton-tipped applicator stick for use w/70% EtOH

S111 (40) (carolina; k3-70-3033; 100/box)

chromatography apparatus

S111 counter

chromatography plates

S111 (38) (carolinal k3-68-9400; per box)

stop watch or timer

see Lab Manager

acetone, 10% in pet ether, (500 ml needed TOTAL)

S111 shelf

erlenmyer flask w/ corks, 500 ml (6 needed)

S111 shelf

corks, size (7); 20.6mm

S111 shelf (carolina; k3-71-2007; 12/pk)

paper clips

S111 (21), see division secretary

pencils

S111 (21), see division secretary

scissors, 2 needed

S111 (35)

millimeter ruler, 2 needed

S111 (21)

sand

S111shelf

mortar and pestle, 8 needed

S205 (29)

spoons, plastic (4 needed)

S111 (16)

small plastic funnels, 4 needed

S111 shelf

disposable pipet, 1ml (10 needed)

S111 (28,29)

rubber stopper w/ hole (size 4)

S111 shelf

disposable pipet, 1mlx 1/100 for rubber stopper

S111 floor

capillary tubes/fine glass tubes

S111 (42)

Whatman #1 chromatography paper

S111 (38) (carolina; k3-68-9110; 100 sheets)

small beaker, 4 needed 

S111 shelf

test tubes, large (25x200)

S111 floor

test tubes, small

S111 (46,47)

test tube rack, 2 needed 

S109 shelf

test tube clamps, 6 needed

S111 (35)

Cheesecloth 

S111 (62) (carolina; k3-71-2690; 12/pk)

container w/ cool water

S205 ICE MACHINE

wash bottle w/ distilled water

S111 counter

Return to Contents Section
 

EXP. 6: MITOSIS AND ASEXUAL REPRODUCTION

 

 

allium root tip slides

Box 13, S

whitefish (coregorius)blastula slides

Box 13

chromosome kit (red and purple beads)

S109 drawer

paramecium in fission slides

Box B

budding yeast slides

Box G

daphnia parthenogenesis slides

Box 13

Mitosis film and projector

S111 (42)

umbrella sedge propagation kit

(carolina; k3-15-7336; per kit)

daphnia, live

(carolina; k3-L520; set/60)

daphnia anatomy, handout

S201 lab folder

begonia, live (Plants)

(carolina; k3-15-7260; each)

african-violet, live (Plants)

(carolina; k3-15-7343; each)

cups, for plants for propagation

S111 (24)

millimeter ruler

S111 (21)

string for chromosomes, (lengh 75cm or 30")

S111 (38)

Denoyer-Geppert Chart # 1893: Plant Mitosis

S110 floor

Return to Contents Section
 

EXP. 7: MEIOSIS: SEGREGATION AND INDEPENDENT ASSORTMENT

 

 

Sordaria Agars (below):

 

sordaria plate, wild

(carolina; k3-15-6291; each)

sordaria plate, gray

(carolina; k3-15-6293; each)

sordaria plate, tan

(carolina; k3-15-6295; each)

crossing agar plates, sordaria

(carolina; k3-15-6353; 10 plates)

cotton-tipped applicator stick

S111 (40) (carolina; k3-70-3033; 100/box)

chromosome slides

Box 17

molded bread (started)

local store

nutrient agar plates

S111 shelf or (carolina; k3-82-1860; 10 plates)

scalpel or knife for molded bread

S111 (35)

forceps for molded bread

S111 (35)

Meiosis film and projector

S203 shelf

chromosome kit

S109 drawer

string for chronosomes, (length 75cm or 30")

S111 (39)

disposable gloves

SRI or carolina or teacher desks: S109 & S110

microscope slides

SEE LAB #1

microscope cover slips (22x22)

SEE LAB #1

Return to Contents Section
 

EXP. 8: THE PROTISTA

 

 

pond water in dropping bottles

(carolina; k3-L25B; class/60)

amoebae, live

(carolina; k3-L1; class/120

amoeba anatomy, handout

S201 lab folder

paramecium, live

(carolina; k3-L2A; class/120)

dinoflagellates, live

(carolina; k3-13-1750; per culture)

microscope slides

SEE LAB #1

microscope cover slips (22x22)

SEE LAB #1

methelene blue stain (Loeffler), in dropping bottles

S111 shelf (carolina; k3-87-5795; 500ml)

innoculation loops

S111 (35)

bacteria type slides

Boxes 26,26A, & B

diatoms prepared slides

Box D

dinoflagellates slides

Box D

marine algae set

(carolina; k3-15-1540; per set)

disposable gloves

SRI or carolina or teachers desks: S109 & S110

10% bleach solution (disinfectant)

S111 sink

Return to Contents Section
 

EXP. 9: FUNGI AND LICHENS

 

 

chlorophyta slides

Box D

pyrrhophyta slides

Box E

rhizopus slides

Box G, I

corprinus slides

Box H

puccinia slides

Box H

lichen slides

Box G2

molded bread

local store

mushrooms, live & slide

Box H & local store

razor blades for cutting mushrooms

instructor desk, S109 & S110

Gill fungus life cycle, handout

S201 lab folder

lichen set & handout

S110 & S201 folder (carolina; k3-15-6400; per set)

baker's yeast for yeast culture

S111 shelf, local store

molasses or sugar for yeast culture

S111 shelf

fermentation tubes, 5 needed /section

S111 shelf

disposable gloves

SRI or carolina or teachers desks: S109 & S110

sterile dropping pipet, cal. 1 ml

S111 floor or teachers desks: S109 & S110 (carolina; k3-21-5840; 100/box)

sordaria (ascus formation w/ sordaria)

carolina; k3-15-5847; per set)

10%glycerine, in dropping bottles

S111 shelf

Return to Contents Section
 

EXP. 10: TRACHEOPHYTE PLANT TISSUES

 

 

root slides

Box S, S2

Denoyer-Geppert Chart # 1901: Roots

S110 floor

stem slides

Box Q, R

Denoyer-Geppert Chart # 1902: Stems

S110 floor

leaf slides

Box P, P2

Denoyer-Geppert Chart # 1903: Leaves

S110 floor

grass, tree branches w/ flowers and leaves from

UVI grounds

Denoyer-Geppert Chart # 1904: Flowers

S110 floor

three plant displays

S110 shelf

lichen set & handout

S110 shelf

Return to Contents Section
 

EXP. 11: PLANT LIFE CYCLES I

 

 

fern slides

Box S2, L

moss thallus slides

Box R, P2

liverwort, slides

Box J

liverwort, live

(carolina; k3-15-6500; per set)

Denoyer-Geppert Chart # 1896: Liverwort

S110 floor

moss, live

(carolina; k3-15-6650; per set)

Denoyer-Geppert Chart # 1897: Moss

S110 floor

Marchantia life cycle, handout

S201 lab folder

Polytrichum life cycle, handout

S201 lab folder

sporophyte fern plant (fern prothalia)

(carolina; k3-15-6881; per culture)

fern life cycle slides & handout

Box L, M (carolina; k3-PB403; per set)

Denoyer-Geppert Chart # 1898: Fern

S110 floor

leaf types set

Box P, P2 (carolina; k3-23-8805; per set)

lilac leaf, slide

Blue Box

Return to Contents Section
 

EXP. 12: PLANT LIFE CYCLES II

 

 

lilac leaf, slide

Blue box

pine leaves

(carolina; k3-15-7130; each)

pine life cycle set

Box N (carolina; k3-PB568; per set)

pine life cycle, handout

S201 lab folder

Denoyer-Geppert Chart # 1899: Gymnosperms

S110 floor

lily life cycle set 

Box O, P (carolina; k3-B5980; per set)

lily life cycle, handout

S201 lab folder

fresh flowers/flower types

Box T (carolina; k3-PB1248; per set)

Bio Viz  Student review Questions, handout

S201 lab folder

fresh fruits/fruit types (PB11.31; 26-884-0 12 Btls)

Box T

razor blades

S111 counter

 Return to Contents Section
 

EXP. 13: IDENTIFYING LOCAL ANGIOSPERMS

 

 

local flowers: Student Tour of UVI Campus

UVI grounds

Monocot-Dicot Comparison set

Box T (carolina; k3-PB949; per set)

Dicot & Monocot Comparison, handout

S201 lab folder

Denoyer-Geppert Chart # 1900: Monocot and Dicot Seed Germination

S110 floor

Return to Contents Section


 


BIO 142: General Biology Manual by Helen Gjessing

 

 

14

15

16

17

18

19

20

21

22

23

24

25

26

27


 

EXP. 14: ANIMAL GROUPS

 

 

TABLE A:

TABLE B:

sponge, jellyfish, sea anemone, brittle star, urchin, sea fan, fluke (slide), tapeworm, rotifer (slide), chiton, snail or conch, squid, earthworm, leech, tick (slide), crayfish, centipede, fly (plastic block), lancet (slide), lamprey, bony fish, frog, bird

sponge, coral, san dollar, starfish, sea cucumber, planaria (slide), sea slug, clam or scallop, octopus, spider, horseshoe crab, barnacle, millipede,butterfly or moth, turnicate: sea squirt, hagfish, shark, lizard, cat or mirror ( to look at human)

TABLE C:

TABLE D:

jellyfish, sponges chalina, echinoderms, sea urchin, flatworms and worms, octopi, shipworms, mollusks, bugs, grasshoppers, shellfish, snakes, lizards, birds, frog, fish, spiders, bivalve, chitone, molluska, leech, human baby, coelenterates, echinodermata, centipede, scorpions, corals, horseshoe crab, lugworm, nereis, oyster, crayfish

brittle star, sand dollar, sea cucumber, ascaris, horseshoe, lamprey, shark, cat

See Denoyer-Geppert Chart #1882: Coelenterates, #1883: Flatworms and Roundworms, #1884: Earthworms, #1885: Crayfish, #1886: Grasshopper, and #1888: 

S110 floor

Label Specimens on Table A: 1A through 30A

Label Specimens on Table B: 1B through 30B

(PREFERRED SET UP FOR LAB 14)

 

TABLE ONE

TABLE TWO

1. Flatworms

1A. fluke
POM960 & Box 3

1B. tapeworm (taenia psiformis) POM965

2. Roundworms

2A. roundworms (ascaris lumbricoides) POM1080

2B. hookworm (ancylostoma caninum) POM1100

3. Arthropods

3A. crayfish

3B. grasshopper or fly in block

4. Echinoderms

4A. urchin

4B. sea star (starfish)
AA-26-1565

5. Cnidarian

5A. sea anemone (metridium)
POM865

5B. sea fan

6. Molluscs

6A. octopus
POM1530

6B. snail (helix)
POM1480

7. Annelids

7A. earthworm (lumbricus terestris) POM1365

7B. leech
POM1401

8. Arthropods

8A. tick or mite
Box 11

8B. horseshoe crab

9. Echinoderms

9A. sea cucumber
P380C

9B. sand dollar

10. Echinoderms

10A. rotifer (slide)

10B. lancet (slide of brochiostoma)

11. Extra

11A. Sponge

11B. human (use mirror)

Return to Contents Section
 

EXP. 15: VERTEBRATE TISSUE

 

 

microscope slides

see lab 1

microscope cover slips (22x22)

see lab 1

normal saline, 0.9 or .85% NaCl

S111 shelf

sterile lancets, (hypolet kit)

S112 (46, 47) (carolina; k3-70-0507; per kit)

hypolet lancets

S112 (46,47) (carolina; k3-70-0508; 100ct)

hypolet guards

S112 (46,47) (carolina; k3-70-0509; 100ct)

prep pads (alcohol preps)

S112 (46,47) (carolina; k3-70-0530; 100ct)

gauze pads

S112 (46,47) (carolina; k3-70-0540; 100ct)

toothpicks, flat ended

S111 (23)

methylene blue stain

S111 (64) (carolina; k3-87-5795; 500ml)

containers w/ disinfectant for used slides, 2 needed

S111 floor

bleach, 10% (disinfectant)

S111 sink

connective tissue slides

Box 20, 20A

bone tissue slides

Box 20, 20A

areolar tissue slides

Box 20, 20A

muscle slides

Box 23

neuron/nervous tissue slides

Box 24

blood cells slides, human & frog

Box 22

epithelial cells slides

Box 21

skin slides

Box 21

Return to Contents Section
 

EXP. 16: CNIDARIANS AND PLATYHELMINTHES

 

 

Mixed Hydra, live

(carolina; k3-L61; 60/class)

Brown Planaria, live

(carolina; k3-L215; 30/class)

Black Planaria, live

(carolina; k3-L220; 30/class)

Cnidarians (jellyfish, hydras, sea anemones, corals)

S110

Flatworms (Platyhelminthes), display case

S110

hydra slides

Box 7

obelia slides

Box 6, 6A

gonionemus slides 

Box 6, 6A

obelia medusae slides

Box 6, 6A

planaria slides c.s.& w.m.

Box 4

taenia tapeworms slides (all stages)

Box 2

pres. fasciola hepatica slides 

Box 3

schistosoma mansoni slides

Box 3A

Denoyer-Geppert Chart # 1883: Flatworms and Roundworms

S110 floor

Return to Contents Section
 

EXP. 17: NEMATODES AND ANNELIDS

 

 

dissecting pans, 25x17x1

S110 floor (carolina; k3-62-9210; each)

ascaris lumbricoides (mostly female)

S112 shelf; (carolina; k3-9265C; 100/set)

lumbricus terestris (earthworms)

S112 shelf; (carolina; k3-P403; 100/set)

ascaris slides (x-sect.)

Box 5

lumbricus slides (x-sect.)

Box 5

earthworm slides, c.s.

Box 10

annelids, display case

S110, Box 10

scalpel or scissors (surgical)

S111 (35)

hand lens 

S111 (35) (carolina; k3-60-2276; 12/pk)

dissecting pins

S111 shelf

Denoyer-Geppert Chart # 1884: Earthworms

S110 floor

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EXP. 18: ARTHROPODS

 

 

grasshoppers

S112 shelf; (carolina; k3-P724C; 12/pk)

Denoyer-Geppert Chart # 1886: Grasshopper

S110 floor

grasshopper mouth slides

Box 13

cambaris (crayfish, damp-packed)

cold room; (carolina; k3-P590D; 12/set)

Denoyer-Geppert Chart # 1885: Crayfish

S110 floor

anthropods, display case

S110

millipede, display case

S110

tweezers

S111 (35)

scissors or scalpels

S111 (35)

hand lens

S111 (35)

dissecting pins 

S111 shelf

dissecting pans

S110 floor

anthropods slides

Box 11

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EXP: 19  MOLLUSCS AND ECHINODERMS

 

 

venus (clams)

cold room; (carolina; k3-P513C; 12/set)

Denoyer-Geppert Chart # 1888: Clam

S110 floor

asterias (starfish)

cold room; (carolina; k3-P331S; 12/set)

clam, display

S110

star, display

S110

mollusc, specimens 

S110

echinoderm,specimens (display cases & jars)

S110

screwdriver

S201

tweezers

S111 (35)

scissors or scalpels

S111 (35)

hand lens

S111 (35)

dissecting pins

S111 shelf

dissecting pans

S110 floor

Return to Contents Section
 

EXP. 20: RESPIRATION AND DIGESTION

 

 

parafin on hot plate (class will heat as needed)

S109

specimen tags/labels; 1/student

S111 (16) (carolina; k3-65-7500; per 100)

double-injected latex frog; 1/student

COLD ROOM; (carolina; k3-P1491A; 12/pack)

Denoyer-Geppert Chart # 1890: Frog Development

S110 floor

Denoyer-Geppert Chart # 1891: Frog Anatomy

S110 floor

benedict's reagent

S111 shelf

IKI soln.

S111 shelf

3 containers to store frogs, 1/lab

S111 floor

medium zip lock bags for frogs, 1/student

S111 (24)

dissecting scissors or scalpels

S111 (35)

hand lens

S111 (35)

dissecting pins/needles

S111 shelf

dissecting pans

S110 floor

X-Jumbo Frog, in large tank of water

(carolina; k3-L1496; 6/set)

Lung Function Model

S110 shelf (carolina; k3-69-2636; each)

Diaphragm and Balloons

S110 shelf (carolina; k3-69-2637; set)

Human Larynx (demonstrates role of epoglottis)

S110 shelf (carolina; k3-56-6913; each)

human torso model

S110 counter

sagittal section human head model,

S110 shelf

wall charts, human resp. system

S110 wall

Frohse Anatomical Chart # FA 8: Digestive System

S110 wall

large test tubes w/ stoppers in test t. rack (40/section)

S111 floor

small test tubes w/ stoppers in test t. rack(16/section)

S111 floor

test tube racks, 4 (place next to hot plates)

S109 next to small incubators

test tube clamps

S111 (35)

pipets, 5ml or 10ml grad.cyl.

S111 (40)

pipet bulbs

S111shelf

hot hands

S111 (6)

oil

S111 shelf

starch

S111 shelf

sugar

S201 shelf

salt pork or bacon, small pieces

local store

egg white, cooked (pea size)

local store

sugarless/rice/dietic crackers

local store

10% HCl in dropping bottle

S111 shelf

lipase, 10% (5 ml/student)

S111 shelf (carolina; k3-87-2500; 25g)

pepsin, 10% (2ml/student)

S111 shelf (carolina; k3-87-9380; 125g)

bile salts, 105 (ml/student)

S111 shelf

distilled water, 1 gallon

S110 counter

hot plates(4), for beakers with water

S111 counter or shelf

large beakers, 4 needed (for hot water bath)

S201 shelf

Return to Contents Section
 

EXP. 21: THE CIRCULATORY SYSTEM

 

 

heart models

S110 shelf

Frohse Anatomical Charts #FA 3: Nervous System and Circulatory System; #FA 4: Schema-Circulation and Heart-Skin


S110 wall

frogs used in lab #20

cold room

Denoyer-Geppert Chart # 1890: Frog Development

S110 floor

Denoyer-Geppert Chart # 1891: Frog Anatomy

S110 floor

dissecting scissors or scalpels

S111 (35)

hand lens

S111 (35)

dissecting pins/needles

S111 shelf 

dissecting pans

S110 floor

Return to Contents Section
 

EXP. 22: THE NERVOUS SYSTEM

 

 

frogs used in lab #21

cold room

spinal cord model

S110

Denoyer-Geppert Chart # 1890: Frog Development

S110 floor

Denoyer-Geppert Chart # 1891: Frog Anatomy

S110 floor

brain/human head model

S110

Frohse Anatomical Chart # FA 3: Nervous System and Circulatory System

S110 wall

neuron slides

Box 24

spinal cord slides, c.s.

Box 24

watch glass or small petri dishes 

S111 (24)

dissecting scissors or scalpels

S111 (35)

hand lens

S111 (35)

dissecting pins/needles

S111 shelf

dissecting pans

S111 floor

forceps

S111 (35)

Return to Contents Section
 

EXP. 23: REPRODUCTION AND EXCRETION

 

 

2 rat, preserved, male

cold or animal room; (carolina; K3-P1875S; each)

1 pregnant female rat

cold or animal room; (carolina; k3-P1875A; each)

2 rat. preserved. female (non-pregnant)

cold or animal room; (carolina; k3-P1875S; each)

x-sect through testis slides

Box 17

ovary w/ follicles, slides

Box 17

ovary w/ corpus luteum, slides

Box 17

human repro system: chart, models, keys

S110

human torso model (oval tubule sect)

S110

Frohse Anatomical Chart # FA 10: Male Pelvis and Female Pelvis

S110 wall

Return to Contents Section
 

EXP. 24: ANIMAL DEVELOPMENT

 

 

sea star/starfish composite slides

Box 14

composite frog development slides

Box 15

pres. stages of frog development

S110

chick development slides

Box 16

frog development models

S110

transparencies of chick development 

S203

models of human embryonic stages

S110

chick stages, plastic blocks

S110

placenta models, pres, & charts

S110

Denoyer-Geppert Chart # 1890: Frog Development

S110 floor

Denoyer-Geppert Chart # 1891: Frog Anatomy

S110 floor

Return to Contents Section
 

EXP. 25: SELECTION

 

 

1000 toothpicks/lab section:

S111 (23)

250 all green

250

500 half green and half plain

500

250 plain

250

green food coloring or dye

S111 shelf

Signs: Biology Lab in Progress

S111 floor

Return to Contents Section
 

EXP. 26: HUMAN GENETICS

 

 

Human Genetics Biokit

(carolina; k3-17-3850; per kit)

Blood group Identification Biokit

(carolina; k3-70-0124; 30/kit)

ABO/Rh Antisera Set

(carolina; k3-70-0222; per set)

small cups, plastic or paper

S111 (24)

drinking water

S208 or local store

sickled rbc slide

Box (22)

Return to Contents Section
 

EXP. 27: FIELD TRIP - SEASHORE LIFE

 

 

NO PREP NEEDED

NO PREP NEEDED

Return to Contents Section


 


NSC 102

LIMIT TO CELL SIZE

 

 

500 ml 2% Agar

S111 shelf

disposable petri dishes or ice tray

S109 floor

color indicator (ex. methelene blue, brom cresol green

S111 shelf

mild acid (acetic, carbonic, or nitrous)

S111, 201/5

stop watch or timer

SEE lab manager (carolina; k3-68-4025; each)

Return to Contents Section
 

OSMOSIS

 

 

osmometer