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salicylic acid 25g |
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acetic anhydride |
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1% aqueous ferric chloride solution in dropping bottles, 1g/100 m |
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sat. aqueous sodium bicarbonate soln. 350 ml, 6.9g/100ml |
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ethyl acetate |
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potassium iodide soln. in dropper bottle, 100 ml (0.25g iodine, 0.5g KI, and 10 ml water are mixed. This solution should be straw-colored in appearance.) |
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phenol 1g |
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aspirin tablet |
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sulfuric acid (conentrated) in dropping bottle |
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hydrochloric acid (concentrated) |
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petroleum ether (30 - 60C) |
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aniline (technical grade, crude, highly colored) 25g |
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acetic anhydride 35ml |
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activated charcoal 8g |
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p-aminophenol 25g |
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acetic anhydride 25 ml |
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sodium acetate tri hydrate 30g |
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activated charcoal 8g |
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hydrochloric acid (conc.) 25ml |
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analgesics; anacin, excedrin (extra strength), tylenol, bufferin |
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B.C. powder, generic aspirin, generic acetaminophen |
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small bottles containing solns of each of the following chemicals dissolved in 50/50 CH2CLs/ EtOH {1 g/20 ml of mixed solvent except aspirin, for which 0.5 g is recommended} will be required: (Prepare immediately prior to lab period) |
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4'-Hydroxyacetanilide (acetaminophen) |
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aspirin (acetylsalicylic acid) |
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caffeine |
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salicylamide |
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20 ml of a combined standard reference mixture of all four compounds at the same conc. |
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chromatography sheets w/ fluorescent indicator, cut into six |
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10 cm x 6.6 cm slides |
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rulers, cm |
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hand-held UV lamp |
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open end capillary tubing, 1 mm |
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filter paper |
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32oz wide mouth, screw cap jars like Mason jars |
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iodine (small amt. in several 32oz developing jars) |
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ethyl actate |
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methylene chloride and ethanol |
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10 old cigars, 1/stud. (buy in advance and allow to air dry) |
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5% aqueous sodium hydroxide soln. 1520ml |
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methanol 125 ml |
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sat. methanolic picric acid 125 ml |
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pine applicator sticks, 1 box |
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fast filter paper (Whatman #1, E and D 617, or S and S 595) |
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ethanol |
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dry tea leaves, 310 g (3/4 lbs) |
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calcium carbonate, powder 125 g |
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magnesium sulfate, anhydrous 25 g |
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methylene chloride 1250 ml acetone 125 ml |
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acetone 125 ml |
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petroleum ether (30 - 60C) 125 ml |
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cotton |
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15 x 125 mm test tubes |
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20 x 150mm sidearm test tubes (flared) |
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fast filter paper (E & D no. 617 or S & S no. 595) |
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buchner funnel |
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salicylic acid 3 g (derivative) |
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toluene 50 ml (derivative) |
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petroleum ether (30 -60C) 15 ml (derivative) |
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ground coffee (regular grind) 1 lb (454 g) |
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calcium carbonate, powder 125 g |
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magnisium sulfate, anhydrous 175 g |
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methylene chloride 1250 ml |
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petroleum ether (30 -60C) 125 ml |
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acetone 125 ml |
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fast filter paper (E & D # 617 or S & S # 595) |
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buchner funnel |
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salicylic acid 3 g (derivative) |
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toluene 50 ml (derivative) |
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petroleum ether (30 -60C) 15 ml (derivative) |
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brominating solution, 65 ml in dropper btl (0.55 g anhyd. sodium acetate powder, 65 ml acetic acid, & 7.4 g Br2) |
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zinc dust 2.5 g |
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methanol 250 ml |
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diethyl ether 500 ml |
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acetic acid, conc. 150 ml |
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sat. aqu. sodium chloride soln. (31.7 g/100 ml water; stored in polyethylene btl) |
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10% (by wgt.) aqu. sodium hydroxide soln (10 g/90 ml water; stored in polyethylene btl) |
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sucrose 500 g |
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baker's yeast; 5 pkgs dry yeast or 200 g cake yeast |
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pasteur salts (2.0 g potassium phosphate, 0.2 g calcium phosphate, 0.2 g calcium phosphate, 0.2 g magnesium sulfate, and 10.0 ammonium tartrate dissolved in 860 ml water) |
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celite 200 g |
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sat. aqu. barium hydroxide 250 ml (8.6 g/100 ml water) |
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kerosene or xylene 75 ml |
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potassium carbonate, anhydrous 500g |
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500 ml erlenmeyer flasks |
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small bottles |
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glass bubing |
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isopentyl (isoamyl) alcohol (3-methyl-1 butanol) 165 ml (136) per 10 students |
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glacial acetic acid (225 ml) 230 g |
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sulfuric acid, conc. 50 ml |
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5% aqu. NaCO3 (sodium bicarbonate) 1200 ml |
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sat'd aqu. NaCl soln. 200 ml (31.7 g/100 ml water; store in polyethylene btl) |
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anhydrous magnesium sulfate 50 g |
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boiling chips, 200 g |
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salicylic acid 110 g |
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methanol 275 g |
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sulfuric acid, conc. 110 ml |
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5% aqu. NaHCO3 625 ml |
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calcium chloride, anhydrous 10 g |
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manometers |
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Y-tubes |
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sodium hydroxide, pellets 125 g |
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methyl salicylate 65 g (? to prepare) |
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1M aqu. sulfuric acid 2L |
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blue litmus paper |
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mossy zinc 150 g |
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6M sulfuric acid 750 ml |
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methyl oleate 65 ml |
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methanol 1.5 L |
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10% Pd on carbon 1.2 g |
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No. 2 one-hole rubber stopper |
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No. 3 one-hole rubber stopper |
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6" rubber tubing: new with no gas leakage |
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12" rubber tubing: new with no gas leakage |
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short (7 in.) disposable pipet |
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5% bromine in CH2Cl2; 100 ml (5 ml Br2:95ml CH2Cl2) PREPARE FRESH |
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methylene chloride 1pt. |
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lard or other fat or oil 125 g |
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sodium hydroxide 30 g |
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95% ethanol 750 ml |
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sodium chloride 625 g |
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glacial acetic acid 120 g |
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chlorosulfonic acid 80 g (place the acid in a large buret in a hood. A pair of rubber gloves should be placed in the hood for use by the students.) |
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lauryl alcohol (1-dodecanol) 125 g (The lauryl alcohol is usually supplied in a narrow mouth bottle. Melt the alcohol (mp 24-27C) and pour the liquid into a wide mouth bottle. When it crystallizes, it is easilly broken into small pieces with a spatuta.) |
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1-butanol 625 ml |
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sodium carbonate 125 g |
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sodium phosphate (Na3PO4.12H2O) 15 g |
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sat. sodium carbondate soln. 250 ml (dissolve 600 g of Na2CO.10H2) in 1L of water) |
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soap solution 125 ml (Add one bar of Ivory soap to 1L of distilled water. Stir the solution occasionally and allow the mixture to stand overnight. Remove the remainder of the bar. The mixture can be used directly.) |
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4% calcium chloride soln. 10ml (dissolve 4 g of calcium chloride in 100 ml of water) |
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oven adjusted to 80C |
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hot plates |
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3-methylbenzoic acid (m-toluic acid) 50g |
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thionyl chloride 55ml (90 g) |
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diethylamine 125 ml (90 g) |
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5% aqu. sodium hydroxide soln.. 500 ml |
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10% aqu. hydrochloric acid soln. 250 ml |
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anhydrous ether 1L |
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anhydrous sodium sulfate 30 g |
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chromatographic columns, 25 mm x 200mm; 5-10 Make columna from 25 mm tubing. Attach a 25 mm x 200 mm test tube to the top and 6 mm glass tubing to the bottom. |
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petroleum ether 1L |
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Alumina, chromatographic grade 300 g |
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manometers (purification by vacuum distillation) |
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Y-tubes (purification by vacumm distillation) |
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pentane 5 ml |
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hexane 5 ml |
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toluene 5 ml |
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m-xylene 5 ml |
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ethanol 5 ml |
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gasoline (10 ml of one type or 5 ml of each brand or grade) |
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gas chromatograph |
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hypodermic syringe (10 microliter or smaller) |
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sample vials or metal containers 10 |
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spices 175g (ground cloves, allspice, ground cumin, and ground cinnamon) |
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methylene chloride 250 ml |
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sodium sulfate, anhyrous 50 g |
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1M sodium hydroxide soln. (40g/L) |
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benzoyl chloride 10 ml |
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semicarbazide hydrochloride 10 g |
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sodium acetate, anhydrous 10 g |
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ethanol, absolute 50 ml |
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methanol 100 ml |
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silica gel G slurry 1 jar (use methylene chlorid-methanol) (2:1) as the solvent in preparing the slurry. Use a 4 oz, wide-mouth, screw-cap har. |
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solution A (100ml): 2g borneol in 100ml diethyl ether |
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solution B (100ml): 10g chromium trixide (CrO3) and 5 g conc. sulfuric acid dissolved in 100 ml water |
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solution C (100 ml): 2 g camphor in100 ml diethyl ether |
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methylene chloride 100 ml |
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methanol 50 ml |
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microscope slides 100 |
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wide-mouth jars (for developing tanks) 10 |
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iodine tanks 2 |
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open end capillary tubing 1 vial |
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rulers with centimeter scale 2 |
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sodium dichromate dihydrate 25 g |
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conc. sulfuric acid 20 ml |
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oxidizing reagent: (2g dichromate:8ml water:1.6ml H2SO4 |
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borneol (racemic) 15 g |
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diethyl ether 425 ml |
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anhydrous magnesium sulfate 25 g |
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methanol 25 ml |
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sodium borohydride 4 g (check purity) |
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5% sodium bicarbonate soln. 125 ml (50 g/1L water) |
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light source (lamp or flashlight) 1 per student |
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hot plate, 70 watt type |
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sublimation apparatus |
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spearmint or caraway seed oil, 30 ml per student |
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glass wool (pr\yrex) |
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fractionating column |
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distilling head |
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stainless steel sponge, king size |
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manometers, one per vacuum setup |
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gas chromatograph |
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polarimeter |
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refractometer |
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2-chlorobutame 10 ml |
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2-bromobutane 10 ml |
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t-butyl chloride (2-chloro-2-methylpropane) 10 ml |
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1-chlorbutane 10 ml |
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crotyl chloride (1-chloro-2-butene) 10ml |
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chloroacetone 10 ml |
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benzyl chloride (alpha-chlorotoluene) 10 ml |
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bromobenzene 10 ml |
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bromocyclohexane 10 ml |
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bromocyclopentane 10 ml |
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(place all in dropper btls & store in the hood; lachrymators) |
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15% (by wgt) sodium iodide in acetone soln. 250 ml 15 g/85 g acetone |
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1% (by wgt) silver nitrate in ethanol soln. 250 ml 1 g/100 g absolute ethanol |
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n-BUTYL BROMIDE: |
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sodium bromide 300g |
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n-butyl alcohol (1-butanol) 225 ml (189 g) |
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conc. sulfuric acid 450 ml |
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sodium hydroxide 5 g |
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t-PENTYL CHLORIDE: |
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t-pentyl alcohol (t-amyl alcohol, 2-methyl-2-butanol 275ml (220g) |
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conc. hydrochloric acid 625 ml |
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5% sodium becarbonate soln 325 ml |
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anhydrous calcium chloride 20 g |
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ammonium chloride 115 g |
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ammonium bromide 210 g |
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sulfuric acid, conc. 360 ml |
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n-butyl alcohol (1-butanol) 60 ml |
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t-butyl alcohol (2-methyl-2-propanol) 60 ml |
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sat. aqu. sodium bicarbonate 125 ml |
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sodium bicarbonate, solid 15 g |
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anhydrous sodium sulfate 10 g |
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crushed ice |
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gas chromatograph |
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hypodermic syringe (1 microliter) |
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refractometer |
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t-Butyl alcohol (2-methyl-2-propanol) 75 ml |
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alpha- phenylethyl alcohol (alpha-methylbenzyl alcohol or secphenethyl alcohol) 75 ml |
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hydrochloric acid, conc. 300 ml |
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5% (by wgt) aqu. sodium bicarbonate 75 ml |
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calcium chloride, anhydrous 50 g |
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acetone, rgt grade, dry 1L |
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ethanol, absolute 1L |
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bromthymol blue indicator 10 ml |
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0.01N sodium hydroxide 2L; (0.40 g/1liter soln.) |
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magnetic stirring bars 5 |
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50 ml burets 5 |
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stopwatches or timers 5 |
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1 ml pipets |
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alcohol samples; 1 ml of each alcohol (provide a choice of ethanol, 1-propanol, 2-propanol, 2-methoxyethanol, 2-chlorethanol, ethylene glycol, or 1-phenylethanol) |
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3.9 M sulfuric acid soln: 150 ml (double if duplicate runs required) |
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0.0196 M potassium dichromate soln; 15 ml (double this amt. if duplicate runs are required) |
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ultraviolet-visible spectrophotometer (1 or more) |
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spectrophotometer cells (at least 2) |
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50 microliter syringe (1 per instrument) |
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timer (calibrated in minutes); 1 per instrument |
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1 ml pipet; 1 per instrument |
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10 ml pipet; 1 per instrument |
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pipet bulb; 1 per instrument |
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cyclohexanol 220 ml (210 g) |
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85% phosphoric acid (14.7 M); 60 ml (100 g) |
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10% aqu. sodium carbonate (100 g/L); 1L |
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anhydrous calcium chloride 35 g |
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sodium chloride (table salt) |
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UNSATURATION TESTS: |
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Br2/CH2Cl2 test solution (2% bromine by volume) 50 ml |
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aqu. KMNO4 soln. (1% wgt/volume)50 ml |
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1,2-dimethoxyethane; small bottle |
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sodium hydroxide 200 g |
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cyclohexene 100 g |
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chloroform (keep in hood) 325 ml |
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benzyltriethylammonium chloride 13 g |
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methylene chloride 650 ml |
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sat. aqu. NaCl soln 375ml(31.7 g/100 ml water) (stored in polyethylene bottle) |
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anhydrous sodium sulfate 25g |
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magnetic stirrers |
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calcium chloride 125 g |
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styrene (in hood) 25 g |
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borane-tetrahydrofuran complex; 70 ml (1.0 M soln in tetrahydrofuran) |
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30% hydrogen peroxide 25 ml |
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3 M sodium hydroxide soln 50 ml |
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diethyl ether 500 ml |
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sat. sodium bicarbonate 250 ml |
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anhydrous magnesium sulfate 15 g |
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wooden applicator sticks 10 |
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10 ml disposable hypodermic syringes (w/ needles) 1 per student |
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rubber septa to fit a ST 19/22 joint (or appropr. size) 1 per student |
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mercuric acetate 100 g |
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styrene 35 g |
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diethyl ether 700 ml |
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6 M sodium hydroxide 160 ml |
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3 M sodium hydroxide 325 ml |
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fresh sodium borohydride 7 g (fresh sodium borohydride must be used. It should also be finely powdered. Place a small amount of powdered material in some mehtanol. It should bubble vigorously if the hydride is active). |
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magnetic stirrers; 1 per student |
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magnetic stirring bars; 1 per student |
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waste container for mercury; 1 per class, in hood |
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gas chromatograph |
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bromobenzene 68 g (45 ml) |
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magnesium turnings 11 g |
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anhydrous ether 500 ml |
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solvent grade ether 200 ml |
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benzophenone 55 g |
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anhydrous sodium sulfate 20 g |
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petroleum ether (30-60C) 650 ml |
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ligroin (60- 90C) 650 ml |
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iodine; small bottle |
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calcium chloride (or drierite) for drying tubes) 50 g |
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bromobenzene 68 g (90 ml) |
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magnesium turnings 11 g |
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anhydrous ether 500 ml |
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solvent grade ether 200 ml |
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dry ice 1/4 lb |
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5% aqu. NaOH 500 ml |
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conc. HCl 150 ml (or 6 M HCl, 600 ml) |
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calcium chloride (drying tubes) 50 g |
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iodine; small bottle |
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mehtylene chloride, 2L |
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magnitic stirrers |
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hot plates with stirrer |
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methyl benzoate 70 g (200 - 300 ml) per 10 students |
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sulfuric acid, conc. 175 ml |
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nitric acid, conc. 50 ml |
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sodium chloride 50 g |
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methanol 250 ml |
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rock salt |
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acetanilide 40 g |
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sulfuric acid, conc. 25 ml |
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mitric acid, conc. 25 ml |
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aqu. ammonium hydroxide, conc. 350 ml |
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ethanol, 95% 1500 ml |
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decolorizing carbon (Norit) 10 g |
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miroscope slides 40 |
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silica gel G slurry 1 jar |
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wide-mouth jars 10 |
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iodine tanks 2 |
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open end cappilary tubes 1 vial |
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rulers with centimeter scale 2 |
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methylene chloride 500 ml |
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dichloromethane (methylene chloride) 1L |
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anhydrous aluminum chloride 175 g (use in hood/noxious vapors) |
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acetyl chloride 100 g |
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hydrochloric acid, conc. 300 ml |
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sat. sodium bicarbonate soln 700ml(6.9 g/100 ml water) |
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anhydrous magnesium sulfate 15 g |
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The following chemicals will be needed in an amount of approx. 10g per student (0.075 mole). Each student will use (or be assigned) on of the chemicals from this list: |
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toluene |
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0-xylene (1,2-dimethylbenzene) |
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m-xylene (1,3-dimethylbenzene) |
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p-xylene (1,4-dimethylbenzene) |
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ethylbenzene |
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mesitylene (1,3,5-trimethylbenzene) |
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cumene (isoproylbenzene) |
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anisole (methoxybenzene) |
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solvents for spectroscopy: |
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carbon tetrachloride 1pt |
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deuteriochloroform 100ml |
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manometers |
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NMR tubes |
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IR salt plates |
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sulfanilic acid, monohydrate 50 g |
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anhydrous sodium carbonate 15 g |
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dimethylaniline 30 ml |
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glacial acetic acid 30 ml |
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10% aqu. sodium hydroxide 1000 ml |
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sodium chloride 120 g |
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sat. aqu. sodium chloride 120 ml (36 g/100 ml water) |
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conc. HCl 60 ml |
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norit |
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pH paper |
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TESTS: |
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Multifiber fabric 10A |
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15% aqu. sodium sulfate 125 ml |
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conc. sulfuric acid |
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sodium carbonate 100g |
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sodium dithionite (sodium hydrosulfite) 50 g |
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Dyes (in small waxed envelopes) per student |
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picric acid 0.5 g |
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indigo powder 0.1 g |
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congo red 0.1 g |
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eosin 0.1 b |
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alizarin 0.1 g |
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methyl orange 0.1 g |
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malachite green 0.1 g |
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pieces of wool yarn (10" long) 36 (one 4 oz. skein of wool yarn will serve about 20 - 25 students. (Natural yarn not white) |
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fabric pieces (or multifiber fabric 10 A): wool, orlon, dacron, nylon, cotton, acetate |
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Each student requieres six pieces of multifiber fabric 10A. If material is used to prepare separate squares (2" x 2") of each fabric, the following approx. amounts of 45" width fabric are required: |
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wool (5 squares/student) = 1/3 yd/20 students |
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cotton (5 squares/student) = 1/3 yd/20 students |
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polyester (3 squares/student) = 1/3 yd/20 students |
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nylon (3 squares/student) = 1/3 yd/20 students |
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(materials should be neutral color, undyed, soft finish) |
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Mordant Baths (1500 ml of each contained in 2L beakers) |
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0.1 M potassium aluminum sulfate (alum) |
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0.1 M cupric sulfate (copper) |
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0.1 M potassium dichrimate (chrome) |
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0.1 M stannous chloride (tin) |
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0.1 M tannic acid |
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0.1 M potassium anitmony tartrate (tartar emetic) |
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(keep mordants warm on steam bath or hot plate. Supply each with a glass rod for mixing. Provide one bath of mordant per 15 students) |
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aluminum foil 1 12" square per student |
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index card 1 |
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string |
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sodium dithionite (sodium hydrosulfite) 0.5 g - 1.0 g |
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sodium hydroxide pellets 1 each |
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10% aqu. sodium carbonate 1 ml |
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10% aqu. sodium sulfate 1 ml |
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p-Nitroaniline 1.5 g |
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10% hydrochloric acid 6 ml |
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sodium nitrite 0.8 g |
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beta-aphthol 0.6 g |
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10% aqu. sodium hydroxide 10 ml |
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biphenyl 0.15 g |
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liquid detergent (like Dreft) |
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l. Paper chromatography of food colors: |
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12cm x 24 cm sheets of whatman no. 1 filter paper |
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32 oz. wide mouth scrow cap jars (or mason jars) |
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1-pentanol (n-amyl alcohol) 350 ml/10 students |
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absolute alcohol 350 ml/10 students |
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food colors:red, blue, yellow, and blue |
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thin wall capillary tubing (open end) |
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microscope slides 1 box |
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Standards (0.1 g/20 ml water): |
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F, D and C red no. 2 amaranth (acid red 27) 25 g |
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F, D and C blue no.1 erioglaucine or brilliant blue 25 g |
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F, D, and C blue no.2 indigo carmine 25 g |
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F, D, and C green no.3 fast green FCF 25 g |
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F, D, and C yellow no.5 tartrazine (acid yellow 23)25 g |
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F, D, and C yellow no. 6 sunset yellow FCF 25 g |
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F, D, and C red no.3 erythrosin B |
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F, D, and C red no.40 allura red |
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II. Paper chromatography of the Dyes from a Powdered Drink Mix of Gelatin Dessert: need stains mentioned above. |
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Powdered drink mix (kool aid or other brand): black cherry, lime, strawberry, cherry, orange grape . lemon-lime, punch, and reapberry (one pkg for class) |
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gelatin dessert (jello or other brand; several pkgs, assorted flavors) |
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III. Separation of Food Colors Using Pre-Prepared TLC Plates: |
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chromatography sheets, 20/box (cut to 5 cm x 10 cm plates) |
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scissors |
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food colors: red, green, yellow, and blue |
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2-propanol (isopropyl alcohol) 100 ml/10 students |
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conc. ammonium hydroxide 25 ml/10 students |
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standard solns of red no. 40 (or red 2), blue no.2, yellow no.5, andy yellow no.6 dissolved in a minimum of water. |
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8 oz. wide-mouth screw cap jars for development thin wall cpillary tubing (open end) to make micropipets |
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IV. Separation of a Dye Mixture Using Hand-Dipped TLC Slides: |
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several jars(4 oz.) containing methylene chloride/silica gel G slurry (3 ml CH2Cl2 per gram silica gel G) |
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methylene chloride (dichloromethame) |
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silica gel G 4 oz. wide-mouth screw cap jars for development chambers |
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filter paper to line development chambers |
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tin wall capillary tubing (open end) dto make micropipets |
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microscope slides 4 -5 per student |
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unknown dye mixture: (all three dissolved in 20 ml methanol) |
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0.1 g erythrosine |
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0.1 g fast green FCF |
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0.1 g sunset yellow |
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Standard dye solutions of single dyes (0.1 g/20 ml methanol) of: |
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FDC Red No. 2; amaranth |
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FDC Red No. 40; allura red |
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FDC Blue No. 2; indigo carmine |
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FDC Freen No. 3; fast green FCF |
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FDC Yellow No. 5; tartrazine |
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FDC Yellow No. 6; sunset yellow |
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FDC Red No. 3; erythrosin B |
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(one btl of 20 ml of each dye will suffice for a very large class.) |
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2-propanol (isopropyl alcohol) 120ml/10 students |
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methanol 30 ml/10 students |
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conc. ammonium hydroxide 25 ml/10 students |
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V. Separation of a Dye Mixture using Pre-Prepared TLC Plates: |
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chromogram sheets: 20 for 160 students or 20 for 80 students |
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(cut to 5 cm x 10 cm plates) |
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4 oz. wide-mouth screw cap jars for development chambers |
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thin wall capillary tubing (open end) to make micropipets |
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unknown dye mixture (see IV) |
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standard dye solns (see IV) |
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2-propanol (isopropyl alcohol) 100 ml/10 students |
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conc. ammonium hydroxide 25 ml/10 students |
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VI. Seperation of a Dye Mixture by Column Chromatography |
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chromatography column made from a 20 cm length of 10 mm glass tubing |
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glass wool |
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white sand |
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tubing to fasten to column exit |
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disposable pipets |
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adsorption alumina, coarse 60 g/10 students |
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2-propanol (isopropyl alcohol) 1 L/10 students |
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conc. ammonium hydroxide 200 ml/10 students |
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ethanol (absolute) 200 ml/10 students |
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methanols 200 ml/10 students |
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unknown dye mixture (part IV) 15 ml/10 students |
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spectrophotometer cells (for UV/Visible spec) at least 2 |
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|
strained spinach (baby food) 120 g |
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95% ethanol (or absolute ethanol) 150 ml |
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methylene chloride 120 ml |
|
glass wool |
|
sodium sulfate, anhydrous 25 g |
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iodine tanks 2 |
|
open end capillary tubing 1 vial |
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rulers with centimeter scale 2 |
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spectrophotometer cells (for UV/Visible spect.) at least 2 |
|
For Column Chromatography: |
|
chromatography columns |
|
ligroin (60 - 90C) 500 ml |
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ethyl acetate 500 ml |
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alumina (acid washed) 125 g |
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white sand 50 g |
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test tubes (small, 10 ml) 12 per student |
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For Thin-Layer Chromatography: |
|
microscope slides 75 |
|
silica gel G slurry 1 jar (use methylene chloride as the solvent in preparing the slurry (3 mlCH2Cl2/gram silica gel G). |
|
wide mouth jars 10 |
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|
95% ethanol 1.5 L |
|
benzaldehyde 190 g (FRESH BOTTLE) |
|
Sodium cyanide 19 g (in hood, use trap) |
|
15% aqu. sodium hydroxide for the gas traps 2 L |
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|
benzaldehyde 250 ml (FRESH BOTTLE) |
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thiamine hydrochloride (vitamin B1) 45 g (fresh btl or stored in refrig) |
|
95% ethanol 1.5 L |
|
2 M aqu. sodium hydroxide 125 ml |
|
Occasionally, the benzoin will form as an oil on cooling. Scratching and cooling will usually induce crystallization. Alternatively, the solution may be heated to redissolve the benzoin and then cooled more slowly. |
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|
benzoin 125g |
|
conc. nitric acid 625 ml |
|
95% ethanol 500 ml |
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|
benzil 70 g |
|
potassium hydroxide, pellets 70 g |
|
95% ethanol 250 ml |
|
decolorizing carbon 10 g |
|
conc. hydrochloric acid |
|
pH paper |
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|
acetanilide 150 g per 10 students |
|
chlorosulfonic acid 425 ml (750 g) per 10 students |
|
sodium hydroxide 15 g per 10 students |
|
mehtylene chloride 2.5 L per 10 students |
|
500 ml separtory funnels per student |
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|
conc. ammonium hydroxide 200 ml per entire class |
|
6 N hydrochloric acid 125 ml per entire class |
|
conc. hydrochloric acid 50 ml per entire class |
|
decolorizing carbon (norit) 50 g per entire class |
|
sodium becarbonate 50 g per entire class |
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|
2-aminothiazole 35 g per entire class |
|
pyridine (dried over KOH pellets) 125 ml per entire class |
|
10% sodium hydroxide 1 L per entire class |
|
6 N hydrochloric acid 250 ml per entire class |
|
95% ethanol 1.5 ml per entire class |
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|
2-aminothiazole 35 g per entire class |
|
pyridine (dried over KOH pellets) 125 ml per entire class |
|
10% sodium hydroxide 1 L per entire class |
|
conc. hydrochloric acid 500 ml per entire class |
|
sodium acetate 125 g per entire class |
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|
enterobacter aerogenes (bacteria slant) |
|
bacillus subtilis, var. niger (bacteria slant) |
|
sulfanilamide or sulfathiazole |
|
distilled water |
|
hot plates |
|
50 ml water and 20 ml 95% ethanol |
|
sulfapyridine 0.25 g |
|
grease pencils |
|
tweezers |
|
bunsen burner |
|
95% ethanol 500 ml |
|
watch glass |
|
filter paper discs (tabs) |
|
filter paper |
|
NUTRIENT AGAR & BROTH |
|
peptone 15 g per group of 3 students |
|
tryptone 18 g per group of 3 students |
|
agar 15 per group of 3 students |
|
sodium chloride 16 g per group of 3 students |
|
glucose 2 g per group of 3 students |
|
inoculating loops 2 per group of 3 students |
|
2 L ml erlenmeyer flasks 4 per group of 3 students |
|
250 ml erlenmeyer flads r per group of three students |
|
disposable plastic petri dishes (150 or 100 x 15mm) 40 |
|
glass "rake", described in part IV 1 |
|
shaker bath (30 - 35C) |
|
autoclave |
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|
ALL SOLNS ARE QUANTITIES FOR 10 STUDENTS |
|
p-toluidine 100 g (200 ml) |
|
hydrochloric acid, conc. 375 ml |
|
decolorizing carbon (norit) 12 g |
|
sodium acetate trihydrate 150 g |
|
actic anhydride 105 ml |
|
magnesium sulfate hydrate 315 g |
|
potassium permanganate 375 g |
|
celite 100 g |
|
ethanol, 95% 15 ml |
|
sulfuric acid (20% by wt. = 21 g H2SO4 (79 ml water) 750 ml |
|
dilute aq. ammonia (35 ml conc. amm. hydroxide/75 ml water) 1.5L |
|
acetic acid, glacial 100 ml |
|
1 L beakers 10 needed |
|
pH paper (8 - 9) |
|
NMR tubes |
|
chloroform-D, 100 ml (in dessicator) |
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|
ALL SOLNS ARE QUANTITIES FOR 10 STUDENTS |
|
p-aminobinzoic acid 65 g |
|
95% ethanol 850 ml |
|
conc. sulfuri acid 65 ml |
|
10% sodium carbonate soln. 750 ml |
|
diethyl ether, solvent grade 1.5 L |
|
magnesium sulfate, anhydrous 40 g |
|
pH paper |
|
methylene chloride 2 L |
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|
ALL SOLNS ARE QUANTITIES FOR 10 STUDENTS |
|
sodium metal (store undre mineral oil/work in hood) 20 g |
|
absolute alcohol, anhydrous, freshly opened 300 ml |
|
powdered anhydrous KI (grind if necessary, dry in oven) 10 g |