BIOCHEMISTRY

CHE 348

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HO. 1: ENZYME KINETICS

acid phosphatase, 1 g

p-nitrophenyl phosphate, 5 g

magnesium chloride

sodium acetate

sodium fluoride

Na2HPO4

KOH

HO. 2: MELTING TEMPERATURE CALF THYMUS DNA

calf thymus DNA, 100 g

deoxyribonuclease, one 10,000 units

HO. 3: BASE CONTENT OF DNA

calf thymus DNA

n-butanol

HO. 4: SPECTROSCOPIC METHODS OF PROTEIN DETERMINATION

Lowry Method:

sodium potassium tartrate

CuSO4.5H2O

Folin-Ciocalteu "phenol reagent", 2N

bovine serum albumin

Warburg Christian Method

Bicincchoninnic acid assay

Biuret Method

sodium potassium tartrate

copper sulfate

NaOH

HO. 5: DETERMINATION OF THE MW OF HEMOGLOBIN BY GEL FILTRATION

hemoglobin, 25 g

myoglobin, 250 mg

bovine serum albumin, 10 g

cytochrome c, 10 mg

blue dextran

sephadex G-75, 50 g

HO. 6: DETERMINATION OF THE ISOELECTRIC POINT OF A PROTEIN

IEF MIX 3.6-9.3

IEF MIX 3.6-6.6

Methyl Red

Ampholytes, 10 ml

amyloglucosidase

trypsin inhibitor

carbonic anhydrase I

carbonic anhydrase II

HO. 7: SDS PAGE GEL ELECTROPHORESIS-MW OF ACID PHOSPHATASE

B-mercaptoethanol

sodium dodecyl sulfate

bisacrylamide

acrylamide

glycine

methanol

trifluoro acetic acid

acetic acid

ammonium persulfate

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WEB SITE PREPARED BY EUGENIA SOMERSALL-SELLO

AND

CHRISTA RICHARDSON (1998 UPWARD BOUND STUDENT)

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