DIVISION OF SCIENCE AND MATH SAFETY TRAINING

Return to the Instructor Page

TEXTBOOKS/PRINTED REFERENCE MATERIALS

AUDIO-VISUAL MATERIALS

MISCELLANEOUS MATERIALS: POSTERS

ON-LINE MATERIALS (INTERNET, ETC.)

LABORATORY SAFETY CHECKLIST

Safety Rules for Refrigerators, Freezers, and the Cold Room

INDEX OF SELECTED SAFETY TOPICS

TEXTBOOKS/PRINTED REFERENCE MATERIALS

MSDS Binders with Individual Chemical Information

CHE151 Safety Rules August 2006

CHE253 Safety Rules August 2006

Chemistry General Lab Safety Rules August 2006

Science Safety Handbook

The Merck Index: 11th Edition

Prudent Practices for Handling Hazardous Chemicals in Laboratories

Prudent Practices for Disposal of Chemicals from Laboratories

Laboratory Safety Manual

Laboratory Safety and Health: ACS Short Course

First Aid Manual for Chemical Accident

Right-to-Know Pocket Guide for Labs

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AUDIO-VISUAL MATERIALS

Chemical Safety Measures, Spills, and Disposal (Video)

Howard Hughes Medical Institute (HHMI) Assessing Risks of Toxic Chemicals (Video) @2004

Howard Hughes Medical Institute (HHMI) Centrifugation Hazards (Video) @2004

Howard Hughes Medical Institute (HHMI) Safety in the Research Laboratory Set One (Video) @2004

Howard Hughes Medical Institute (HHMI) Safety in the Research Laboratory Set Two (Video) @2004

Howard Hughes Medical Institute (HHMI) Chemical Storage Hazards (Video) @2004

Science Lab Safety @2004

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MISCELLANEOUS MATERIALS: POSTERS

Laboratory Techniques Poster by Denoyer-Geppert

Laboratory Safety Poster by Denoyer-Geppert

CPR for Adults (Ages 9 and Over)

Hazardous Material Identification Guide

MSDS Training Poster

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ON-LINE MATERIALS (INTERNET, ETC.)

Biology Student Safety Information: http://www.uvi.edu/SandM/biolab.htm

Chemistry Student Safety Information: http://www.uvi.edu/SandM/chemlab.htm

Laboratory Instructor Web Site: http://www.uvi.edu/SandM/instruct.htm

Howard Hughes Medical Institute: http://www.hhmi.org/

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LABORATORY SAFETY CHECKLIST

The following checklist may used to:

Determine whether or not a safe environment exists.
Indicate possible areas of concern and danger.
Act as a monitoring device for periodic safety checks.
Act as a permanent record of an on-going safety program.

1. General good housekeeping prevails and aisles are clear of materials and apparatus.

2. Adequate storage space is provided for chemicals, materials, and apparatus.

3. The classroom/laboratory has no blind spots where students cannot be supervised by the teacher from anywhere in the classroom/laboratory.

4. Open shelves are equipped with lips or restraining wires to prevent spilling of chemicals or broken glassware during explosion or earthquake.

5. Separate fireproof waste containers are provided for spent matches, filter paper.

6. The light level is adequate (about 75 to 100 foot – candles at work surfaces).

7. Separate waste containers are provided for broken glass, non-flammable used chemicals, and so on.

8. A fire extinguisher is kept in working condition at all times in a conspicuous and accessible place.

9. An approved fire blanket is kept in a conspicuous and accessible place.

10. Flammable fluids and materials stored in the classroom are kept in fireproof containers (not glass) and in quantities sufficient for only one day’s supply.

11. An approved fire-retardant storage cabinet separate from the classroom is used to store large quantities of flammable, corrosive, and other dangerous chemicals.

12. Flammable liquids are not kept in refrigerators, unless it is certified as explosion proof.

13. Food is not kept in refrigerators used for storing science supplies.

14. To avoid contact with possible flooding or other spilled chemicals, store large storage bottles of strong acids and bases plus other dangerous chemicals on the lowest cabinet shelves but not on the floor.

15. Ethers are periodically disposed of if the presence of peroxides is suspended.

16. Sodium is stored under kerosene or oil.

17. Incompatible chemicals are not stored adjacent to one another.

18. All chemicals are dated on receipt, and current inventory maintained.

19. Quantities of hazardous chemicals kept on hand are limited to the amounts needed during one school year.

20. Proper labels and signs are kept in place on all chemicals and on the storage area.

21. Neutralizing reagents are located conveniently in the laboratory for treatment of spills.

22. Chemical containers are inspected periodically for leakage or deterioration (such as sediments and discoloration), and approved disposal procedures are followed as necessary.

23. Any gas cylinder is stored according to the required safety code, e.g., chained or strapped in a cart or to the wall.

24. Splash-proof safety goggles, face shields, aprons, and so on are available in a cart or to the wall.

25. Eye wash fountains and safety showers are easily accessible, identified, and flushed weekly to remove rust and scale.

26. Fume hoods are clean, uncluttered, and tested periodically for adequate air flow.

27. Electrical outlets and extension cords are kept in a safe working condition.

28. All electrical equipment should be three-wire grounded except for double-insulated tools or equipment which has no exposed metallic parts that could become energized.

29. Electrical equipment such as the refrigerator and aquarium aerators are connected directly to wall outlets and not serviced through an extension cord.

30. Gas outlets and burners are maintained in safe working condition.

31. The location of the matter gas shut-off valve is known and readily accessible.

32. Plumbing fixtures are in correct operating condition. Faucets must be equipped with vacuum breakers to prevent backflow.

33. Sink drains are inspected periodically for corrosion and kept free from solid wastes.

34. Fire drill regulations are posted and familiar to all students.

35. The Department of Science and Math’s emergency procedures are prominently posted.

36. An adequate first-aid kit, including the Red Cross Standard First Aid and Personal Safety Manual or appropriate alternate information, is provided.

37. The teacher is familiar with first aid and safety measures pertinent to science instruction and as presented in DSM Laboratory Safety Manuals and reference materials. The MSDS, DSM Laboratory Safety Manual, Chemical Hygiene Plan and Science Safety Handbook are readily accessible.

38. Classroom/Laboratory free of irregularities or dangerous situations including but not limited to faulty equipment, proper ventilation, and safety supplies.

39. Written materials relating to safety precautions provided on a regular basis as follow-up and reinforcement to the regular teaching and instructional programs.

40. Students informed verbally and in written form as to the consequences of inappropriate behavior, horseplay, and unsafe use of equipment and materials. Waiver slips signed by students which warns them of specific dangers for unsafe use of materials or equipment.

41. Documentation of the types of instruction and the dates when safety-related instructions were demonstrated, conducted, or test completed and files maintained.

42. Potential dangers (safety hazards, defective equipment, or unsafe conditions) reported immediately to the designated safety officer. Students are not allowed to work under dangerous situations. Instructors do not allow students to continue working in a dangerous situation.

43. Instructors qualified and able to show proper use and care and maintenance of safety equipment. Documentation of staff training (group or individual) maintained. Documentation and recording of the types of safety materials, in-service training, and instruction classes that staff members have been sent to or attended.

44. Division of Science and Math and instructors have an emergency plan of action that includes the following:

Safety procedure plan which defines the who, what, when, and where involved, and how emergency help will be handled and managed.
Location and procedures that will be utilized by medical specialists, appropriate for the specific injury.

45. Full disclosure (specific listing of potential dangers) on file.

46. Safety record-keeping includes:

Scripts
Videotapes/films
Lessons
Test for knowledge and training
Accidents and procedures to remediate (includes dates and times or meetings.)
Equipment inspection
Emergency card documents (medical release forms)
Training (e.g., CPR, OSHA, guides and guidelines) for both students and staff to indicate knowledge of current change and procedures.
First-aid training and kit maintenance
Procedure for contacting the following community resources/information established and made available to every instructor: 1) fire department, 2) ambulance, 3) hospital, 4) doctor/dentist, 5) student medical release form, and 6) student medical records (e.g., special medical problems).

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DRAFT UVI LAB SAFETY CHECKLIST

General Safety

	Sign with Emergency Telephone Numbers call list and NFPA hazard warning labels are posted at lab entrance(s)
	UVI Laboratory Safety Manual is kept in the lab
	A Chemical Spill Kit is available in each lab housing liquid chemicals and Emergency spill procedures posted
	A First-Aid Kit is readily accessible
	A Fire Extinguisher of the appropriate class and size is available
	Safety Shower and Eyewash Unit are readily accessible to lab personnel
	Airfoils and slots on chemical fume hoods are unobstructed; side panels are in place
	Storage of chemicals and equipment in chemical fume hoods is kept to a minimum
	Vacuum pumps are equipped with a belt and pulley guard, if applicable
	Lab equipment is safely operated and maintained according to manufacturers directions

Personal Protective Equipment (PPE), Housekeeping, and Hygiene:

	PPE e.g., gloves, safety glasses/goggles, lab coats, thermal protection, etc. is available (stored clean and in good repair) and worn for the activities being conducted and adherence of the procedure that all PPE is removed before leaving the labs
	Hearing protection is worn for high noise areas (e.g. sonicators, grinders; blowers)
	Full coverage shoes with good sole grips are worn in the lab; no open-toed shoes (sandals) are allowed
	Lab personnel are aware that contact lenses should not be worn in the labs
	Respirator wearers are trained, fit tested, and registered with EHS Coordinator
	Walkways and safety equipment are clear of obstructions
	Work surfaces and benches are free of clutter to reduce risk of spills and accidents
	Spills are cleaned up promptly
	Mechanical pipetting devices are used; pipetting by mouth is not allowed
	Food and beverages are not permitted in the lab working area, or stored in lab refrigerators/freezers
	Refrigerators, freezers, and microwaves are clearly labeled, e.g., "non-flammable storage only" and "no food or beverages
	When unattended, the laboratory is locked at all times to ensure laboratory security and Issue keys to appropriate laboratory users only and make them aware of security requirements

Chemical Safety:

	Proper storage procedures are followed for all chemicals including labeling, capping/sealing, and proper container type/condition
	Liquid chemicals are stored below shoulder height
	Proper dating/storage/use/disposal procedures are followed for peroxide forming compounds, e.g. ethers, THF, ally alcohols

Lab waste disposal:

	Lab personnel are trained before they first begin working with hazardous substances or processes, or equipment
	Outdated and unused chemicals are tagged for removal and then disposed of in the appropriate manner
	All hazardous waste containers are properly sealed and labeled
	A Hazardous Waste Satellite Accumulation Area is designated and the requirements are posted
	Biological Waste placed in proper containers, decontaminated in the appropriate manner, and disposed of properly
	Broken glass is separated from regular waste stream and placed into special containers properly labeled “Broken Glass”
	Needles, razor blades, and small sharps are disposed of in red plastic ‘sharps’ box

Compressed gases:

	Compressed gas cylinders are adequately secured (even when empty) and stored by compatibility
	Gas cylinders are securely transported using a hand truck
	UVI Compressed Gas Rules are posted in a prominent location
	Cylinders are stored away from heat sources
	The regulator connection is leak tested after installation and before each use
	Cylinders with no regulators must be capped (even when empty)
	Contents of cylinders must be clearly labeled
	Cylinders that have not been stored more than 7 years must be removed

Electrical Safety:

	Access to circuit breaker panel is unobstructed
	Openings on breaker panel, receptacle boxes, etc. are sealed
	Ground Fault Circuit Interrupters (GFCI) are used near sinks and wet areas
	Electrical cords are checked periodically for fraying or damage

Fire Safety:

	A Fire Extinguisher of the correct class and size is located near or in the lab
	Large metal drums of flammable liquids are grounded during transfer
	A maximum of 10 gallons of flammable liquids are stored in a lab; the excess is stored in a safety cabinet or safety cans up to a maximum total of 50 gallons
	Flammable liquids over 1 gallon are stored in safety cans
	Sprinkler heads are not blocked
	Vents on flammable storage cabinets are sealed

Drug Enforcement Agency (DEA) controlled substances:

	DEA permit is current (A DEA permit is required for controlled substances)
	DEA use log and employee questionnaires are current for controlled substances on hand
	Controlled substances are stored in secure location
	Outdated or unwanted DEA substances are disposed of appropriately

Biological Materials:

	Bio-Safety Level (BSL) class 2 safety manual kept in the lab
	State and/or federally guidelines for regulated bio-hazardous materials and non-regulated bio-hazardous materials
	Acute toxins (LD50 of £ 100 m g/kg) usage logbook

Record keeping:

	Chemical Hygiene Plan
	Material Safety Data Sheets (MSDS)
	A recent (within the last year) chemical inventory
	DEA records
	Bloodborne Pathogen Program/Exposure Control Plan
	Records of health and safety training for employees and students and safety manuals e.g., Laboratory Safety Manual, Biological Safety Manual, Chemical Waste Management Guide, etc.

Dated: June 2005

Safety Rules for Refrigerators, Freezers, and the Cold Room

1. These are shared resources primarily for support of teaching labs and courses. Storage of food and drink in these units is forbidden at all times.

2. Keep units clean. When a chemical substance is spilled, clean immediately with water. When potentially infectious material is spilled, clean immediately with either 70% isopropyl alcohol or some other disinfectant agent. Afterwards, wipe down area with soap and water.

3. Label all containers that holds experimental material with:

	(for exampleJ
Name	Dr. Maynard
Date	10/15/00
Research	Cell Biology
Expiration	Date

4. Label all containers that holds student material with:

	(for exampleJ
Name	N. Jacobs
Date	3/8/00
Course #	BIO 142
Instructor	Dr. Fujimori
Expiration	Date

5. Do not clutter storage space. Place all glassware that contains cultures and chemicals inside some type of labeled container with a firmly fixed cover. Do not use measuring volumetric flasks and graduated cylinders to store materials. Do not use glass breakers as lids for bottles.

6. Do not carelessly stack material too high upon each other. Especially, do not stack loose etri-dishes plates. Always tape together stacks of plates or place in a plastic bag. Untaped stacks are a potential hazard if they topple when the door is opened or hit accidentally.

7. Place large containers and large bottles on the bottom shelves. Positioned and secured these items wisely so that they will not fall out of the unit.

8. NO FLAMMABLE OR EXPLOSIVE MATERIALS CAN BE STORED IN refrigerator and freezer. FLAMMABLE OR EXPLOSIVE MATERIALS CAN BE STORED IN THEIR PROPER CONTAINER INSIDE THE COLD ROOM ONLY.

9. Refer to either the Handbook of Chemistry or the Sigma-Aldrich catalog as a guide for the list of chemicals that either can be stored or must be stored in a refrigerated area. If no information is available or if there are any problems, please consult the Lab Manager.

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INDEX OF SELECTED SAFETY TOPICS

A – TOPICS

TOPIC	REFERENCE BOOK	LOCATION & PAGE
Accidents and Spills	Chemical Safety Measures, Spills, and Disposal	S203: Video
Acid Spills	Laboratory Safety Manual	S203: 23
Animal Care and Handling	Laboratory Safety Manual	S203: 16
Application for Extremely Hazardous Waste Disposal Permit	Science Safety Handbook	S203: 146
Assembly Bill 2185	Science Safety Handbook	S203: 112
Assembly Bill 3820	Science Safety Handbook	S203: 115

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B – TOPICS

TOPIC	REFERENCE BOOK	LOCATION & PAGE
Basic Handling and Storage Principles	Laboratory Safety Manual	S203: 33
Biological Safety	Laboratory Safety Manual	S203: 15
Biological Science Laboratory Regulations and Student’s Statement	Science Safety Handout	S203: 135

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C – TOPICS

TOPIC	REFERENCE BOOK	LOCATION & PAGE
Cal-OSHA Registration Form for Possession of Carcinogenic Chemicals	Science Safety Handout	S203: 151
Caustic Alkali and Ammonia Spills	Laboratory Safety Manual	S203: 23
Chemical Carcinogens	Laboratory Safety Manual	S203: 18
Chemical Disposal	Laboratory Safety Manual	S203: 34
Chemical Disposal	Chemical Safety Measures, Spills, and Disposal	S203: Video
Chemical Disposal	Prudent Practices for Disposal of Chemicals from Laboratories	S203: Book
Chemical Hazards	Chemical Hazards	S203: Film Loop
Chemical Hygiene Plan	http://www.uvi.edu/SandM/chp.htm	Computer: Internet
Chemical Precautions	Laboratory Safety Manual	S203: 65
Chemical Profiles	Working Safely with Chemicals in the Laboratory	S203: 63
Chemical Safety Guidelines	Laboratory Safety Manual	S203: 6
Chemical Safety Measures	Chemical Safety Measures, Spills, and Disposal	S203: Video
Chemical Spill Procedures	Laboratory Safety Manual	S203: 22
Chemical Spills	Chemical Safety Measures, Spills, and Disposal	S203: Video
Chemical Storage	Laboratory Safety Manual	S203: 31
Chemicals, Handling of Hazardous	Prudent Practices for Handling Hazadous Chemicals in Laboratories	S203: book
Chemistry Laboratory Legal Safety Checklist	Science Safety Handbook	S203: 141
Clean Air Act	Working Safely with Chemicals in the Laboratory	S203:51
Clean Water Act	Working Safely with Chemicals in the Laboratory	S203: 52
Clothing Fires	Laboratory Safety Manual	S203: 48
Comprehensive Environmental Response, Compensation, and Liability Act	Working Safely with Chemicals in the Laboratory	S203: 53
Compressed Gas Safety: Proper Use of Gas Cylinder	Laboratory Safety Manual	S203: 37
Compressed Gas Storage	Laboratory Safety Manual	S03: 38
Corrosive Chemicals Storage Cabinet Blueprint	Science Safety Handbook	S203: 143

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DIVISION OF SCIENCE AND MATH SAFETY TRAINING

CONTENTS

TEXTBOOKS/PRINTED REFERENCE MATERIALS

AUDIO-VISUAL MATERIALS

MISCELLANEOUS MATERIALS: POSTERS

ON-LINE MATERIALS (INTERNET, ETC.)

LABORATORY SAFETY CHECKLIST

The following checklist may used to:

Safety Rules for Refrigerators, Freezers, and the Cold Room

Return to Contents Section

INDEX OF SELECTED SAFETY TOPICS

A – TOPICS

B – TOPICS

C – TOPICS

D – TOPICS